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Mmole; in 0.5 ml of 0.9% NaCl solution ; . DNA was extracted with hot 5% trichloroacetic acid 19 ; , quantified by the diphenylamine procedure 5 ; , and assayed for radioactivity, after being mixed 1: 4 ; with Aquasol New England Nuclear, Cambridge, Mass. ; in a Model 314 Packard Tri-Carb liquid scintillation spectrometer. This method is rapid and reproducible but may be open to the criticism that the DNA has not been rigorously purified 10, 16, 20 ; . To evaluate the method, we conducted parallel assays on portions of the same livers by the hot trichloroace tic acid method just mentioned and by the following procedure. Nuclei were isolated from liver homogenates in 2% citric acid, washed with either 2% acetic acid or 0.25 M sucrose: 0.0015 M CaCl2: 0.006 M Tris, pH 7.4, and then processed according to the recent method of Schneider and Greco 20 ; , except that hot trichloroacetic acid was substi tuted for perchloric acid in the final extraction step. The extract was assayed for DNA specific activity as in the 1st method. As shown in Table 2, the 2 methods were in reasonable agreement; any but minor alterations in DNA labeling should be reliably evaluated by the original shorter, hot trichloroacetic acid method. RESULTS AND DISCUSSION.
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Cooperation Trimbos Institute Utrecht Abstract In 2001 a new law was passed allowing a judge place highly criminal drug addicts in a compulsory 2 year program aiming at the reduction of criminality: the Penal Care Facility for Drug Addicts SOV ; . The SOV is indicated for the hard-core criminal addicts who have a longstanding history of drug addiction with criminal activities. Education, work experience and social skills training are part of the program. Political support for this new facility was conditional on the fact that the SOV starts with a limited number of places and its effects on reduction of criminal behaviour, improvement of addictive behaviour and social integration are evaluated. If the evaluation is positive the SOV will be implemented in more regions of Holland; if the evaluation is negative the SOV will be ended. The effect on addictive behaviour, criminal activities and social integration are evaluated using two control groups. Keywords Drug addiction, compulsory treatment, criminality, quasi-coercive treatment Funding Dutch Ministry of Justice and celebrex.

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